Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1(Genebank accession No.:EU417815) was cloned from mycoparasite fungi Olpitrichum tenellum.OPK1 has an open reading frame of 2 031 bp interrupted by two introns(108 bp,84 bp) and putatively encodes a protein of 676 aa.Phylogenetic analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in fungi.Southern blot analysis indicated that OPK1 is present as a single copy in the genome.RT-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.
三个小麦品种331、抗倒680和鲁麦23经氯化汞、水杨酸或核黄素处理后，叶片中的β-1，3-葡聚糖酶活性均有不同程度的升高。氯化汞处理24h对品种331该酶活性的诱导作用最强。因此取用氯化汞处理24h的331小麦叶片研磨得到粗酶液。将粗酶液经硫酸铵分级沉淀、Phenyl-Sepharose Fast Flow疏水层析、DEAE-Sepharose Fast FloW阴离子交换层析和SephacrylS-100分子筛层析，得到了SDS-PAGE凝胶电泳谱带单一的β-1，3-葡聚糖酶样品。经SDS-PAGE(12％)和凝胶过滤层析，测得其分子量约为52.0～53.6kD。抗菌试验测定显示，纯化的β-1，3-葡聚糖酶对供试的4种病原真菌的生长、孢子萌发或芽管伸长都有一定程度的抑制作用。
