Using primers complementary to the conserved sequence previously published of BTV genomic dsRNA segment 7,a part of the 5′end of segment 7 was synthesized and amplified by RT PCR method.We adapted this method to test on 12 blood samples suspected to be with BTV and the blood sample of a health sheep as a negative control.The results suggested that eleven of the twelve samples were positive,one was negative.In order to compare the sensitivity and reliability of RT PCR technique,Vero cells were used to isolate BTV from these suspected samples and the results will be a control.The study proved that RT PCR is a rapid,sensitive and precise method for detection and identification of bluetongue virus in clinical samples,animal quarantine and scientific research.
The research of the cooperative antivirus activities was carried out using amantadine, ribavirin and herby houttuynia. The inhibitory effect of CPE caused by influenza virus A\-3 in MDCK cell and the therapeutic effect on BALB/c mice pneumonia caused by influenza virus FM1 were examined and analyzed when amantadine, ribavirin and herby houttuynia were used alone or associatively. The results show that the concentrations of amantadine,ribavirin and herby houttuynia with same effect were 256, 512 and 1024 times cooperatively as many as alone in vitro, respectively.The ablities of treating pneumonia of mice when three kinds of drugs were used cooperatively were far higher than those alone in BALB/c mice.
