The shoot-tips of Euonymus fortunei cv.‘Yuanban’excised from healthy in vitro plants were successfully cryopreserved with the vitrification technique. After the shoot tips formed roots and were successfully transferred to soil of outside with the control plant. To study the adaptability to summer high temperatures of the regenerated plantlets, they were tested for vegetative growth (plant height, branch number, branch length, leaf number), and physiological parameters (photosynthetic pigment content, praline and electrolyte leakage) at intervals. The results showed that no statistical difference in the physiological parameters between the regenerated plantlets and the control. The changes in vegetative growth of the regenerated plantlets were quite similar to the control and there was no statistical difference in amount about branch number, branch length, leaf number. However, data of the plant height revealed that the plant height of the control was higher than that of the regenerated ones. The reason for that was not clear and more researches were needed.
