Objective To observe clinical effect of acupuncture and moxibustion on inducing ovulation. Methods Forty cases of anovulatory infertility were treated with electroacupuncture and moxibustion at Zigong (子宫 EX-CA 1), Guanyuan (元关 CV 4) and Zhongji (中极 CV 3) as well as Linggui Bafa therapy, 30 min each time, once a day for 15 days. One menstrual cycle made a session, and totally 3 sessions were required. The level of follicle stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2), the growth and development situation of follicle and endometrium before and after treatment, spontaneous pregnancy rate during follow-up visit were observed and compared. Results After the treatment, the level of FSH, LH and Ez were signifiacntly increased (all P〈0.05), the thickness of endometrium and diameter of follicle were obviously increased (both P〈0.05). As for the spontaneous pregnancy rate during follow- up visit, 9 cases were cured, 25 cases were effective, 6 cases were failed and the total effective rate was 85.0%. Conclusion The acupuncture and moxibustion has better clinical efficacy for anovulatory infertility.