To discuss the inhibiting mechanism of water extraxt solution from Rhodiola on theTyrosinase activity.Tyrosinase inhibiting rate is determined and Lineweaver-Burk curve is made by determining the tyrosinase activity before and after drug（water extract solution） interference oxidant of method,on which we deduce the inhibiting types of the drug.The water extract solutioncan inhibit the tyrosinase activity remarkably with the half inhibiting concentration IC50 3.9g/L.The water extract solution can inhibitthe tyrosinase activity and it is thenoncompetitive inhibitor of tyrosinase.
Based on the sequence analyzing of the cloned gene Dunaliella salina UDP-glucose Dehydrogenase(DsUGD),it shows that the largest open reading frame is 1452bp,the coding protein belongs to UDP-glucose/GDP-mannose dehydrogenase family.The predicted transmembrane regions structure and signal peptides of the DsUGD show that it has one transmembrane structure and may be excretive.The results of the advanced structure analysis of DsUGD shows that it has a high identity with the previous verdict.
