Objective To observe influence of moxibustion at ＂Shenshu＂ （肾俞BL 23） and ＂Zusanli＂ （足三里 ST 36） on proteins expression of Ras, ERKI/2, and Raf in synovial tissues in rats with rheumatoid arthritis （RA） to elucidate molecular mechanisms of moxibustion on treating RA. Methods Thirty Wistar rats were randomly divided into a normal control group （Group Cont）, a model group （Group Mod） and a moxibustion group （Group Mox） with 10 rats in each group. The model was established by using both environmental factors such as wind, cold and damp and a biological factor （Freund＇s complete adjuvant）. Suspended moxibustion was applied at bilateral BL 23 and ST 36 for 20 min, applied once daily for 15 days, starting from the 3rd day after modeling. Volumes of metaleg digiti pedis on the right side were measured before, after and 15 days after modeling. Protein expressions of Ras, ERK 1/2, p-ERKI/2 and Raf in synovium tissues were detected by western blot. Results After modeling, volumes of metaleg digiti pedis on the right side were significantly enlarged, compared with those of rats in Group Cont （P〈0.01）. After treatment by moxibustion for 15 days, the metatarsale girth of metaleg digiti pedis on the right side in Group Mox was significantly reduced, compared with that in Group Mod （P〈0.01）. Protein expressions of Ras, Raf, ERK1/2 and p-ERK1/2 in Group Mod were significantly increased, compared with those in Group Cont （P〈0.01） and Group Mox （P〈0.01）. Protein expressions of Ras, Raf, ERK1/2 and p-ERK1/2 in Group Mox were significantly lowered, compared with those in Group Mod （all P〈0.01）. Conclusions Moxibustion can suppress excessive protein expressions of Ras, Raf and ERK1/2 in synovium tissues of rats with experimental RA to inhibit abnormal activation of Ras-MAPK signal Pathway, which may be one of the molecular mechanisms of moxibustion in alleviating synovitis.
目的探讨手术前后提肛训练对吻合器痔上黏膜环切术(procedure for prolapse and hemorrhoids,PPH)患者术后并发症的影响效果。方法以我院2015年7月至2016年6月间收治的60例行PPH的患者作为对照组,采用常规护理和健康宣教,2016年7月至2017年6月间收治的60例行PPH的患者作为试验组,在对照组的基础上指导其术前2d和术后2周每天进行提肛训练。比较两组患者术后尿潴留的发生率、首次大便排出时间、肛门切口疼痛评分。结果试验组患者术后尿潴留的发生率明显低于对照组(P<0.01),首次大便排出时间较对照组缩短(P<0.05),首次排便、术后第7天和第14天时肛门切口疼痛评分均低于对照组(P<0.05)。结论手术前后提肛训练能够降低PPH术后患者尿潴留的发生率,缩短患者首次大便排出时间,缓解患者肛门切口疼痛。
