Objective To observe the therapeutic effects of post-stroke hand motor impairment treated with acupuncture at Zhongzhu (中渚 TE 3) and Waiguan (外关TE 5). Methods Sixty cases of post-stroke hand motor impairment were randomly divided into an observation group and a control group, 30 cases in each one. The conventional medication, physical therapy, occupational therapy and other rehabilitation trainings were applied in both groups. In observation group, Zhongzhu (中渚 TE 3) and Waiguan (外关TE 5) were punctured. The assessment was performed with neurologie deficit scoring of the National Institutes of Health Stroke Scale (NIHSS), hand motor function, walking ability and the modified Barthel index scale of the Activities of Daily Living (ADL) in both groups before and after treatment. Results After treatment, the scores of hand function, walking ability, ADL and NIHSS were improved in both groups as compared with those before treatment (all P〈0.01). The improvements of hand function, walking ability and ADL :in observation group were superior to those in control group (all P〈0.05). Conclusion The conventional medication and rehabilitation trainings combined with acupuncture at Zhongzhu (中渚 TE 3) and Waiguan (外关TE 5) ean obviously improve hand motor function, walking ability, the activities of daily living and rehabilitation efficacy in the patients with post-stroke hand motor impairment.