Triethyl Phosphite is the intermediate of producing nervous toxin of organic phosphate,which is involved in the chemicals of schedule 3. in Convention On The Prohibition Of The Development,Production,Stockpiling And Use Of Chemical Weapons And On Their Destruction.A method for the determination of triethyl phosphate was established by GC-MS/SIM.Average recovery was 96.3%-111.5% with linear range between concentration and peak area varying from 0.546-10.928 mg/L and the detection limits were 0.011 ng.
The orientation of melittin binding to liposomes with of without transmembrand potential was investigated.With The combination of high performance liquid chromatography and mass spectromeny, we detected The membrane association state of melittin by analyzing its proteolytic products .We found that The enzyme could access ail The proteolyticc sites on protein binding to liposome without membrane potential.while one proteolytic site on The N-terminal of The Protein was blocked de it binding to liposome with negative transmembrane potential The results show that melittin reorients in The latter case.
