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王晓娜

作品数:2 被引量:2H指数:1
供职机构:天津大学化工学院更多>>
发文基金:天津市自然科学基金国家自然科学基金更多>>
相关领域:医药卫生轻工技术与工程更多>>

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HPLC determination of Nimodipine in plasma with an improved sample refining method and its application in pharmacokinetic studies被引量:2
2016年
In order to prepare samples for HPLC analysis with maximum drug recovery and impurity elimination, a revised method for the extraction and purification of a target substance from plasma was developed and applied in a pharmacokinetic study with Nimodipine as a model drug. After protein precipitation of a plasma sample using pure methanol and evaporation of the supernatant to dryness, methanol of various concentrations from 10% to 100% were used to dissolve the remaining residues with the goal of maximizing drug recovery and impurity elimination. Through rigorous screening with HPLC peaks from residual impurity and recovered drug as the criteria, a methanol concentration of 30% was chosen. The standard curve was linear (r2〉 0.999) over the range of 2-160 ng/mL with a limit of quantification (LOQ) of 2 ng/mL. Intra- and inter-day precision values were below 15%, and the accuracy ranged from -1.70% to 5.88% at all three quality control (QC) levels. The wavelength of maximum absorption was 238 nm, and a smaller LOQ value of 2 ng/mL was achieved compared with the reported method. The revised method was successfully applied in a pharmacokinetic study of Nimodipine in rats and sample preparations of lidocaine hydrochloride.
王晓娜陈瑞莲刘文利龚俊波王永莉魏振平
关键词:NIMODIPINEHPLC
微生物合成乳酸的细胞工厂构建研究进展
2024年
乳酸目前广泛应用于食品、医疗和化妆品行业,并因其作为可生物降解塑料聚乳酸的原料而备受关注。微生物发酵合成乳酸具有光学纯度高和底物成本低等优点,因此,研究人员利用合成生物学和代谢工程技术构建了新型微生物细胞工厂,以实现乳酸的高效合成。在此过程中,代谢通路的构建与优化、氧化还原平衡的调节、底物谱的拓宽以及细胞工厂鲁棒性的提高等方面是需要考虑的关键问题。文章详细介绍了微生物合成乳酸的细胞工厂构建研究进展,并对当前面临的挑战和未来的研究方向进行了探讨,旨在为工业微生物高效合成乳酸提供借鉴。
王晓娜田开仁吴昊吴昊乔建军乔建军
关键词:乳酸合成生物学代谢工程鲁棒性
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