[Objective] This study aimed to develop an efficient SDS-PAGE method suitable for analyzing the total protein of Solanum lycopersicum seedling roots.[Method] By using the Solanum lycopersicum seedling roots as the experimental material,the protein extraction,sample volume and other factors influencing the final electrophoresis pattern were respectively optimized.[Result] The high-resolution,clear and reproducible SDS-PAGE pattern could be obtained when the protein was extracted with modified TCA-acetone precipitation and the sample volume for electrophoresis was 60 μg.[Conclusion] This optimized method could basically meet the requirements for study the proteome of tomato seedling roots.
