多色基因组原位杂交(multi-color genomic in situ hybridization,McGISH)鉴定结果表明,MTPP-58有58条染色体,其中11条染色体来自玉米,28条染色体来自四倍体多年生大刍草,17条染色体来自指状摩擦禾和2条玉米与四倍体多年生大刍草易位的染色体。2012-2013年对MTPP-58的饲草产量、品质、越冬率以及繁殖特性研究表明,第1年鲜、干草产量分别为93.845t/hm2和22.083t/hm2,第2年鲜、干草产量分别为82.851t/hm2和14.394t/hm2;粗蛋白(CP)为10.48%,干茎叶比为0.71,越冬率为100%。MTPP-58下部茎干扦插成活率为51.69%,分株成活率为95.50%。多种属聚合远缘杂交创制的MTPP-58聚合了玉米、大刍草、摩擦禾的优良特性,植株根系发达,生长繁茂,分蘖和抗寒性强,生产性能优,可无性繁殖,是一种多种属间杂交创制的新型多年生饲草。
Considering the ecological environment of Sichuan area, an experiment was conducted to breed new maize varieties with high yield, high quality, multi re- sistance and wide adaptability. According to the principle of gene recombination, the authors combined the local tropical germplasm with PB germplasm, which was rich in disease-resistant, lodging-resistant and drought-tolerant genes, and the integration was used as the innovative strategy for breeding. Inbred-line ＇LSC 107＇ was suc- cessfully bred and the new maize hybrid ＇Wande No.l＇ was bred by crossing ＇LSC 107＇ with ＇Yi 99-19＇. The results showed that the maize hybrid had good performance, including high yield, good stability, high quality, excellent disease re- sistance and lodging resistance, and wide adaptation. So it was adaptable for plant- ing in plain and hilly areas of Sichuan Province. With hybrid recombination between local tropical germplasm and PB inbred lines, the inbred-line had high general com- bining ability and strong adaptability, and the hybrid had high yield, high quality and wide adaptability, and had broad prospects of popularization and application.
