A new virus detection method was developed by combining hybridization capture and real-time PCR technology with the sample of Tomato ringspot nepovirus.High purified DNA or RNA was crucial for the real-time PCR detection as the inhibitors of PCR reaction in template and might cause the false-negative cases.A probe was covalent bound to the surface of PCR tube for the DNA capture followed by real-time PCR detection.The steps of nucleic acids preparation and detection were both done in one the same tube.This method reduced the possibility of contamination as well increased the detection efficiency.It can be applied in plant virus detection especially for the sample with many inhibitors of PCR reaction.
