目的 初步探讨hbv基因s区的准种特点与乙型肝炎病毒(hbv)感染临床转归的关系.方法 选择慢性hbv携带者、慢性乙型肝炎患者、慢性重型肝炎患者各3例,所有患者均为男性,且hbv基因型均为c型.扩增患者血清中hbv基因s区片段并克隆,每份样本挑选20个克隆进行测序,并用spss 15.0软件进行统计分析.结果 慢性hbv携带者以及慢性乙型肝炎患者的hbv基因s区准种复杂度小于慢性重型乙型肝炎患者,但差异无统计学意义(t=1.7,p=0.26).对于hbv s区t细胞表位的第45、47和85位氨基酸,慢性乙型肝炎患者的准种构成情况比hbv携带者复杂(p=0.01),与慢性重型乙型肝炎的差别无统计学意义(p=0.06),计算机模拟分析提示优势克隆和非优势克隆的t细胞表位均能与ctl受体有效结合.结论 hbv基因s区部分t细胞表位的准种构成差异可能与hbv慢性感染的临床转归相关.
abstract：
objective to investigate the association of hepatitis b virus(hbv) s gene quasispecies with the outcome of hbv infection.methods serum samples were collected from three chronic hbv carriers, three chronic hepatitis b and three chronic severe hepatitis b patients.all subjects were male and with hbv genotype c.hbv s gene was amplified, and 20 clones of hbv fragment were randomly selected and sequenced from each sample.spss 15.0 software was adopted for analysis.results quasispecies complexity of hbv s gene in chronic hbv carriers and chronic hepatitis b tended lower than that of the severe chronic hepatitis b, but the difference was not of statistical significance (p＞0.05).in t cell epitope 45, 47, 85 amino acid sites of the hbv s gene, the constitution of quasispecies in the chronic hepatitis b was more complex than that of the hbv carriers (p=0.01), but compared with the severe chronic hepatitis, the difference was not significant (p=0.06).the computer model showed that both the dominant clones and the non dominant clones could effectively bind to the receptors of cy
