为了研究茉莉酸介导的基因对根结线虫侵染的响应,以番茄野生型和茉莉酸缺失突变体为试材,采用数字表达谱技术检测分析根结线虫侵染后主茎韧皮部中的差异表达基因。结果表明,茉莉酸缺失突变体中有2 427个基因差异表达,其中1 390个差异基因可富集到112个KEGG(Kyoto Encyclopedia of Genes and Genomes)通路。18个差异基因涉及茉莉酸合成前体α-亚麻酸代谢,100个差异基因参与植物和病原体的相互作用。
[Objective] This study aimed to develop an efficient SDS-PAGE method suitable for analyzing the total protein of Solanum lycopersicum seedling roots.[Method] By using the Solanum lycopersicum seedling roots as the experimental material,the protein extraction,sample volume and other factors influencing the final electrophoresis pattern were respectively optimized.[Result] The high-resolution,clear and reproducible SDS-PAGE pattern could be obtained when the protein was extracted with modified TCA-acetone precipitation and the sample volume for electrophoresis was 60 μg.[Conclusion] This optimized method could basically meet the requirements for study the proteome of tomato seedling roots.
